MURC Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Anti-MURC Antibody (N-term) at 1:1000 dilution + human heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
MURC Antibody (N-term) (Cat. #AP5646a) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MURC Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, WB, E
Primary Accession	Q5BKX8
Other Accession	NP_001018126.1
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	41899 Da
Antigen Region	87-116 aa

Additional Information
Gene ID	347273
Other Names	Muscle-related coiled-coil protein, Muscle-restricted coiled-coil protein, MURC
Target/Specificity	This MURC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 87-116 amino acids from the N-terminal region of human MURC.
Dilution	IHC-P~~1:100~500 WB~~1:1000 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	MURC Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	CAVIN4 (HGNC:33742)
Synonyms	MURC
Function	Modulates the morphology of formed caveolae in cardiomyocytes, but is not required for caveolar formation. Facilitates the recruitment of MAPK1/3 to caveolae within cardiomyocytes and regulates alpha-1 adrenergic receptor-induced hypertrophic responses in cardiomyocytes through MAPK1/3 activation. Contributes to proper membrane localization and stabilization of caveolin-3 (CAV3) in cardiomyocytes (By similarity). Induces RHOA activation and activates NPPA transcription and myofibrillar organization through the Rho/ROCK signaling pathway (PubMed:18332105).
Cellular Location	Cytoplasm, myofibril, sarcomere {ECO:0000250\|UniProtKB:A2AMM0}. Cytoplasm {ECO:0000250\|UniProtKB:A2AMM0}. Cytoplasm, cytosol {ECO:0000250\|UniProtKB:A2AMM0}. Cell membrane, sarcolemma {ECO:0000250\|UniProtKB:A2AMM0}. Membrane, caveola. Cell membrane. Note=In cardiomyocytes, accumulates in the Z-line of the sarcomere. In vascular smooth muscle cells, detected diffusely throughout the cytoplasm. Localizes in the caveolae in a caveolin-dependent manner. {ECO:0000250\|UniProtKB:A2AMM0}

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.