癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
TRIM50 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IHC-P, FC, E |
|---|---|
| Primary Accession | Q86XT4 |
| Other Accession | NP_835226.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 54728 Da |
| Antigen Region | 354-383 aa |
| Gene ID | 135892 |
|---|---|
| Other Names | E3 ubiquitin-protein ligase TRIM50, 632-, Tripartite motif-containing protein 50, TRIM50, TRIM50A |
| Target/Specificity | This TRIM50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 354-383 amino acids from the C-terminal region of human TRIM50. |
| Dilution | WB~~1:1000 IHC-P~~1:100~500 FC~~1:10~50 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | TRIM50 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TRIM50 (HGNC:19017) |
|---|---|
| Synonyms | TRIM50A |
| Function | E3 ubiquitin-protein ligase that ubiquitinates Beclin-1/BECN1 in a 'Lys-63'-dependent manner enhancing its binding to ULK1 (PubMed:29604308). In turn, promotes starvation-induced autophagy activation. Also interacts with p62/SQSTM1 protein and thereby induces the formation and the autophagy clearance of aggresome-associated polyubiquitinated proteins through HDAC6 interaction (PubMed:22792322). Also promotes NLRP3 inflammasome activation by directly inducing NLRP3 oligomerization independent of its E3 ligase function (By similarity). |
| Cellular Location | Cytoplasm Note=Localizes mainly into discrete cytoplasmic punctuate structures heterogeneous in size and shape containing polyubiquitinated proteins |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
TRIM50 belongs to the TRIM/RBCC family. It contains 1 B box-type zinc finger, 1 B30.2/SPRY domain and 1 RING-type zinc finger. TRIM50 is an E3 ubiquitin-protein ligase.
REFERENCES
Micale, L., et al. Eur. J. Hum. Genet. 16(9):1038-1049(2008)
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Cat# AP5637b
