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DAGLB Rabbit pAb
DAGLB Rabbit pAb
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Application 
| E |
|---|---|
| Primary Accession | Q8NCG7 |
| Reactivity | Mouse |
| Predicted | Human, Rat, Pig, Rabbit, Sheep |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 73732 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human DAGLB |
| Epitope Specificity | 51-150/672 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SUBCELLULAR LOCATION | Cell membrane. |
| SIMILARITY | Belongs to the AB hydrolase superfamily. Lipase family. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | KCCR13L, is a 672 amino acid multi-pass membrane protein that belongs to the AB hydrolase superfamily. DAGL beta uses calcium as a cofactor to catalyze the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), a reaction that is required for axonal growth and for retrograde synaptic signaling at mature synapses. DAGL beta functions at an optimal pH of 7 and its activity is inhibited by p-hydroxy-mercuri-benzoate and HgCl2, but not PMSF. There are three isoforms of DAGL beta that are produced as a result of alternative splicing events. |
| Gene ID | 221955 |
|---|---|
| Other Names | Diacylglycerol lipase-beta, DAGL-beta, DGL-beta, 3.1.1.116, KCCR13L, PUFA-specific triacylglycerol lipase, Sn1-specific diacylglycerol lipase beta, DAGLB |
| Dilution | ELISA=1:5000-10000 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| Name | DAGLB |
|---|---|
| Function | Lipase that catalyzes the hydrolysis of arachidonic acid (AA)-esterified diacylglycerols (DAGs) to produce the principal endocannabinoid, 2-arachidonoylglycerol (2-AG) which can be further cleaved by downstream enzymes to release arachidonic acid (AA) for cyclooxygenase (COX)-mediated eicosanoid production (PubMed:14610053). Preferentially hydrolyzes DAGs at the sn-1 position in a calcium- dependent manner and has negligible activity against other lipids including monoacylglycerols and phospholipids (PubMed:14610053). Plays a key role in the regulation of 2-AG and AA pools utilized by COX1/2 to generate lipid mediators of macrophage and microglia inflammatory responses. Also functions as a polyunsaturated fatty acids-specific triacylglycerol lipase in macrophages. Plays an important role to support the metabolic and signaling demands of macrophages (By similarity). |
| Cellular Location | Cell membrane; Multi-pass membrane protein |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
KCCR13L, is a 672 amino acid multi-pass membrane protein that belongs to the AB hydrolase superfamily. DAGL beta uses calcium as a cofactor to catalyze the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), a reaction that is required for axonal growth and for retrograde synaptic signaling at mature synapses. DAGL beta functions at an optimal pH of 7 and its activity is inhibited by p-hydroxy-mercuri-benzoate and HgCl2, but not PMSF. There are three isoforms of DAGL beta that are produced as a result of alternative splicing events.
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