癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Tex14 Polyclonal Antibody
Purified Rabbit Polyclonal Antibody (Pab)
- 产品详情
- 实验流程
Application 
| IHC-P, IHC-F, IF, ICC, E |
|---|---|
| Primary Accession | Q8IWB6 |
| Reactivity | Rat, Dog |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 167901 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human Tex14 |
| Epitope Specificity | 101-200/1497 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SUBCELLULAR LOCATION | Cytoplasm. Midbody. Chromosome, centromere, kinetochore. Note=Detected in the intercellular bridges that connect male germ cell daughter cells after cell division. |
| SIMILARITY | Belongs to the protein kinase superfamily. Contains 3 ANK repeats. Contains 1 protein kinase domain. |
| SUBUNIT | Interacts with KIF23 and RBM44. Interacts with CEP55; inhibiting interaction between CEP55 and PDCD6IP/ALIX and TSG101. |
| Post-translational modifications | Phosphorylated on Thr residues by CDK1 during early phases of mitosis, promoting the interaction with PLK1 and recruitment to kinetochores. Phosphorylated on Ser-437 by PLK1 during late prometaphase promotes the rapid depletion from kinetochores and its subsequent degradation by the APC/C complex. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | TEX14 is a 1,497 amino acid protein that belongs to the protein kinase superfamily and is expressed in testis. The gene encoding TEX14 is located on chromosome 17 and is required for spermatogenesis and normal structure of the intercellular bridge that connects spermatocytes and spermatogonia. TEX14 co-localizes with the centralspindlin complex, MKLP-1 (mitotic kinesin-like protein 1) and male germ cell Rac GTPase (Rac GTPase-activating protein) and converts these midbody matrix proteins into stable intercellular bridge components. TEX14 contains 3 ANK repeats and 1 protein kinase domain. Three isoforms exist due to alternative splicing events. |
| Gene ID | 56155 |
|---|---|
| Other Names | Inactive serine/threonine-protein kinase TEX14, Protein kinase-like protein SgK307, Sugen kinase 307, Testis-expressed sequence 14, Testis-expressed sequence 14 protein, TEX14, SGK307 |
| Target/Specificity | Detected in testis. |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,ELISA=1:5000-10000 |
| Format | 0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| Name | TEX14 |
|---|---|
| Synonyms | SGK307 |
| Function | Required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges. Also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment. Has no protein kinase activity in vitro (By similarity). |
| Cellular Location | Cytoplasm. Midbody. Chromosome, centromere, kinetochore. Note=Detected in the intercellular bridges that connect male germ cell daughter cells after cell division. |
| Tissue Location | Expression restricted to testis. |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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