Anti-CD99 Antibody

Immunohistochemical analysis of CD99 staining in human ewing's sarcoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Additional Information
Gene ID	4267
Other Names	MIC2; MIC2X; MIC2Y; CD99 antigen; 12E7; E2 antigen; Protein MIC2; T-cell surface glycoprotein E2; CD99
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within human CD99. The exact sequence is proprietary.
Format	Mouse IgG. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	CD99
Synonyms	MIC2, MIC2X, MIC2Y
Function	Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).
Cellular Location	Membrane; Single-pass type I membrane protein

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.