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Anti-PALLD Antibody

Western blot analysis of PALLD expression in Hela (A), A375 (B), mouse muscle (C), mouse kidney (D) whole cell lysates.
Immunohistochemical analysis of PALLD staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Primary Accession	Q8WX93
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	150564 Da

Additional Information
Gene ID	23022
Other Names	KIAA0992; Palladin; SIH002; Sarcoma antigen NY-SAR-77
Target/Specificity	Recognizes endogenous levels of PALLD protein.
Dilution	WB~~1/500 - 1/1000
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	PALLD
Synonyms	KIAA0992
Function	Cytoskeletal protein required for organization of normal actin cytoskeleton. Roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. May function as a scaffolding molecule with the potential to influence both actin polymerization and the assembly of existing actin filaments into higher-order arrays. Binds to proteins that bind to either monomeric or filamentous actin. Localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Different isoforms may have functional differences. Involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation. Involved in targeting ACTN to specific subcellular foci.
Cellular Location	Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cytoplasm, myofibril, sarcomere, Z line. Cell projection, ruffle. Cell projection, podosome {ECO:0000250\|UniProtKB:P0C5E3}. Cell projection, lamellipodium. Cell projection, axon {ECO:0000250\|UniProtKB:P0C5E3}. Cell projection, growth cone {ECO:0000250\|UniProtKB:P0C5E3}. Note=Localizes to stress fibers and Z lines (PubMed:11598191, PubMed:16125169, PubMed:17322171, PubMed:17537434). Preferentially expressed in the excitatory presynaptic terminals (By similarity). {ECO:0000250\|UniProtKB:P0C5E3, ECO:0000269\|PubMed:11598191, ECO:0000269\|PubMed:16125169, ECO:0000269\|PubMed:17322171, ECO:0000269\|PubMed:17537434}
Tissue Location	Detected in both muscle and non-muscle tissues. High expression in prostate, ovary, colon, and kidney. Not detected in spleen, skeletal muscle, lung and peripheral blood lymphocytes (at protein level). Protein is overexpressed in FA6, HPAF, IMIM-PC2, SUIT-2 and PancTu-II sporadic pancreatic cancer cell lines

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol