Desmin Antibody

Purified Rabbit Polyclonal Antibody (Pab)

Western blot analysis of Desmin expression in A375 (A), rat heart (B) whole cell lysates. (Predicted band size: 53 kD; Observed band size: 54 kD)
Immunohistochemical analysis of Desmin staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Desmin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

产品详情
实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, ICC
Primary Accession	P17661
Reactivity	Human, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	53 KDa

Additional Information
Other Names	Desmin, DES
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Desmin. The exact sequence is proprietary.
Dilution	WB~~1:1000 IHC-P~~1:50~200 ICC~~N/A
Format	0.01M PBS, pH 7.2, 0.09% (W/V) Sodium azide, Glycerol 50%
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.

REFERENCES

Li Z.,et al.Gene 78:243-254(1989).
Li Z.,et al.J. Biol. Chem. 266:6562-6570(1991).
Vicart P.,et al.Hum. Genet. 98:422-429(1996).
Goldfarb L.G.,et al.Nat. Genet. 19:402-403(1998).
Li D.,et al.Circulation 100:461-464(1999).

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¥ 1,500.00

Cat# AP51162

Size:

50 µl

100 µl

Price:

¥ 1,500.00

¥ 2,600.00

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Availability: 5-7 days

询价

Desmin Antibody

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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