癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Phospho-CHK1(S317) Antibody
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- 产品详情
- 文献引用 : 1
- 实验流程
- 背景知识
Application 
| DB, WB, IHC-P, E |
|---|---|
| Primary Accession | O14757 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Other Names | Serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1, CHEK1, CHK1 |
|---|---|
| Target/Specificity | This CHK1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S317 of human CHK1. |
| Dilution | DB~~1:500 WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Phospho-CHK1(S317) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The S. pombe Chk1 gene encodes a protein kinase that is required for the DNA damage checkpoint. Antibodies against CHK1 recognized a 54-kD protein on immunoblots of mammalian cell extracts. However,CHK1 is modified in response to DNA damage. In vitro, CHK1 directly phosphorylated a regulator of CDC2 tyrosine phosphorylation, CDC25C. In response to DNA damage, CHK1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-cyclin B complex and mitotic entry. CHK1 directly phosphorylates CDC25A during an unperturbed cell cycle, and that phosphorylation of CDC25A by CHK1 is required for cells to delay cell cycle progression in response to double-strand DNA breaks.
REFERENCES
Kramer, A., et al., Nat. Cell Biol. 6(9):884-891 (2004).
Xu, X., et al., J. Biol. Chem. 279(33):34091-34094 (2004).
Ng, C.P., et al., J. Biol. Chem. 279(10):8808-8819 (2004).
Chen, M.S., et al., Mol. Cell. Biol. 23(21):7488-7497 (2003).
Groth, A., et al., EMBO J. 22(7):1676-1687 (2003).
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