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APRT Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - APRT Antibody (C-term) AP2893b
    All lanes : Anti-APRT Antibody (C-term) at 1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 20 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - APRT Antibody (C-term) AP2893b
    APRT Antibody (C-term) (Cat. #AP2893b) western blot analysis in MCF-7 cell line and rat kidney tissue lysates (35ug/lane).This demonstrates the APRT antibody detected the APRT protein (arrow).
  • 14 - APRT Antibody (C-term) AP2893b
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with APRT Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession P07741
Reactivity Human, Rat, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 19608 Da
Antigen Region 143-170 aa
Additional Information
Gene ID 353
Other Names Adenine phosphoribosyltransferase, APRT, APRT
Target/Specificity This APRT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 143-170 amino acids from the C-terminal region of human APRT.
Dilution IHC-P~~1:100~500
WB~~1:1000
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAPRT Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name APRT (HGNC:626)
Function Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.
Cellular Location Cytoplasm.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

A conserved feature of APRT is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis.

REFERENCES

Silva,C.H., et. al.,J. Biomol. Struct. Dyn. 25 (6), 589-597 (2008)
Di Pietro,V., et. al., Clin. Biochem. 40 (1-2), 73-80 (2007)

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