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APRT Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - APRT Antibody (N-term) AP2893a
    APRT Antibody (N-term) (Cat. #AP2893a) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the APRT antibody detected the APRT protein (arrow).
  • 3 - APRT Antibody (N-term) AP2893a
    Fluorescent image of A549 cell stained with APRT Antibody (N-term)(Cat#AP2893a/SA091009AF).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with APRT primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).APRT immunoreactivity is localized to Cytoplasm and Nucleus significantly.
  • 14 - APRT Antibody (N-term) AP2893a
    Formalin-fixed and paraffin-embedded human hepatocarcinoma with APRT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - APRT Antibody (N-term) AP2893a
    Flow cytometric analysis of HepG2 cells using APRT Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC, IF, WB, E
Primary Accession P07741
Reactivity Human, Rat, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 19608 Da
Antigen Region 12-40 aa
Additional Information
Gene ID 353
Other Names Adenine phosphoribosyltransferase, APRT, APRT
Target/Specificity This APRT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-40 amino acids from the N-terminal region of human APRT.
Dilution IHC-P~~1:100~500
FC~~1:10~50
IF~~1:10~50
WB~~1:1000
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAPRT Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name APRT (HGNC:626)
Function Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.
Cellular Location Cytoplasm.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

A conserved feature of APRT is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis.

REFERENCES

Silva,C.H., et. al.,J. Biomol. Struct. Dyn. 25 (6), 589-597 (2008)
Di Pietro,V., et. al., Clin. Biochem. 40 (1-2), 73-80 (2007)

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