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PCNA Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

Western blot analysis of PCNA Antibody (C-term) (Cat.#AP2835b) in Jurkat, Hela, 293 cell line lysates (35ug/lane). PCNA (arrow) was detected using the purified Pab.
Western blot analysis of PCNA (arrow) using rabbit polyclonal PCNA Antibody (C-term) (Cat. #AP2835b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PCNA gene.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PCNA antibody (C-term) (Cat. #AP2835b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of PCNA Antibody (C-term) (Cat#AP2835b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescent analysis of PCNA Antibody (C-term) (Cat#AP2835b) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
PCNA Antibody (C-term) (Cat. #AP2835b)immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCNA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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实验流程
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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IF, E
Primary Accession	P12004
Other Accession	P04961, P61258, P57761, Q3ZBW4
Reactivity	Human
Predicted	Bovine, Hamster, Monkey, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Antigen Region	231-261 aa

Additional Information
Other Names	Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA
Target/Specificity	This PCNA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231-261 amino acids from the C-terminal region of human PCNA.
Dilution	WB~~1:1000 IHC-P~~1:100~500 IF~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	PCNA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.

REFERENCES

Wang,Y., J. Cell. Biochem. 106 (3), 409-413 (2009) Maga,G., Proc. Natl. Acad. Sci. U.S.A. 105 (52), 20689-20694 (2008) Acharya,N., Proc. Natl. Acad. Sci. U.S.A. 105 (46), 17724-17729 (2008)

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¥ 699.00

Cat# AP2835b

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¥ 699.00

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PCNA Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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