TNMD Antibody

Purified Rabbit Polyclonal Antibody (Pab)

AP22288a staining TNMD in human cerebellum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Anti-TNMD Antibody at 1:2000 dilution + Mouse adipose tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	Q9H2S6
Reactivity	Human, Rat, Mouse
Host	Rabbit
Clonality	polyclonal
Isotype	Rabbit IgG
Calculated MW	37130 Da

Additional Information
Gene ID	64102
Other Names	Tenomodulin, TeM, hTeM, Chondromodulin-1-like protein, ChM1L, hChM1L, Chondromodulin-I-like protein, Myodulin, Tendin, TNMD, CHM1L
Target/Specificity	This TNMD antibody is generated from a rabbit immunized with a recombinant protein of human TNMD.
Dilution	WB~~1:2000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	TNMD Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	TNMD
Synonyms	CHM1L
Function	May be an angiogenesis inhibitor.
Cellular Location	[Isoform 1]: Membrane; Single-pass type II membrane protein. Nucleus envelope [Isoform 3]: Cytoplasm.
Tissue Location	Highly expressed in hypovascular connective tissues such as tendons. Also has strong expression in adipose tissue

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

May be an angiogenesis inhibitor.

REFERENCES

Yamana K.,et al.Biochem. Biophys. Res. Commun. 280:1101-1106(2001).
Shukunami C.,et al.Biochem. Biophys. Res. Commun. 280:1323-1327(2001).
Cros N.,et al.Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases.
Brandau O.,et al.Dev. Dyn. 221:72-80(2001).
Clark H.F.,et al.Genome Res. 13:2265-2270(2003).

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¥ 1,250.00

Cat# AP22288a

Size:

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Price:

¥ 1,250.00

¥ 2,050.00

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询价

TNMD Antibody

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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