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OVGP1 Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

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Overlay histogram showing Hela cells stained with AP22205a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22205a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
AP22205a staining OVGP1 in human fallopian tube tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
All lanes : Anti-OVGP1 Antibody (N-Term) at 1:2000 dilution Lane 1: human placenta lysate Lane 2: human uterus lysate Lane 3: human ovary lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 75 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, E
Primary Accession	Q12889
Other Accession	P36718
Reactivity	Human
Host	Rabbit
Clonality	polyclonal
Isotype	Rabbit IgG

Additional Information
Other Names	Oviduct-specific glycoprotein, Estrogen-dependent oviduct protein, Mucin-9, Oviductal glycoprotein, Oviductin, OVGP1, MUC9, OGP
Target/Specificity	This OVGP1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 107-137 amino acids from human OVGP1.
Dilution	WB~~1:2000 IHC-P~~1:100~500 FC~~1:25 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	OVGP1 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Binds to oocyte zona pellucida in vivo. May play a role in the fertilization process and/or early embryonic development.

REFERENCES

Arias E.B.,et al.Biol. Reprod. 51:685-694(1994).
Jaffe R.C.,et al.Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases.
Gregory S.G.,et al.Nature 441:315-321(2006).
Sjoeblom T.,et al.Science 314:268-274(2006).

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¥ 699.00

Cat# AP22205a

Size:

25 ul

50 µl

100 µl

200 µl

400 µl

Price:

¥ 699.00

¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

¥ 4,550.00

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Availability: In Stock

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OVGP1 Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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