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FN3KRP Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 14 - FN3KRP Antibody (N-Term) AP21419a
    AP21419a staining FN3KRP in human Stomach sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - FN3KRP Antibody (N-Term) AP21419a
    All lanes : Anti-FN3KRP Antibody (N-Term) at 1:1000-1:2000 dilution Lane 1: U-87 MG whole cell lysates Lane 2: 293T-17 whole cell lysates Lane 3: NCI-H1299 whole cell lysates Lane 4: Jurkat whole cell lysates Lane 5: mouse kidney lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q9HA64
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 34412 Da
Additional Information
Gene ID 79672
Other Names Ketosamine-3-kinase, 271-, Fructosamine-3-kinase-related protein, FN3K-RP, FN3K-related protein, FN3KRP
Target/Specificity This FN3KRP antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 24-58 amino acids from human FN3KRP.
Dilution WB~~1:1000-1:2000
IHC-P~~1:100~500
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFN3KRP Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FN3KRP {ECO:0000303|PubMed:15137908, ECO:0000312|HGNC:HGNC:25700}
Function Ketosamine-3-kinase involved in protein deglycation by mediating phosphorylation of ribuloselysine and psicoselysine on glycated proteins, to generate ribuloselysine-3 phosphate and psicoselysine-3 phosphate, respectively (PubMed:14633848, PubMed:15137908). Ribuloselysine-3 phosphate and psicoselysine-3 phosphate adducts are unstable and decompose under physiological conditions (PubMed:14633848, PubMed:15137908). Not able to phosphorylate fructoselysine (PubMed:14633848).
Tissue Location Widely expressed; except in skeletal muscle where it is expressed at very low level (PubMed:15331600). Expressed in erythrocytes (PubMed:15137908).
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Phosphorylates psicosamines and ribulosamines, but not fructosamines, on the third carbon of the sugar moiety. Protein- bound psicosamine 3-phosphates and ribulosamine 3-phosphates are unstable and decompose under physiological conditions. Thus phosphorylation leads to deglycation.

REFERENCES

Collard F.,et al.Diabetes 52:2888-2895(2003).
Wiemann S.,et al.Genome Res. 11:422-435(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Collard F.,et al.Biochem. J. 382:137-143(2004).
Oppermann F.S.,et al.Mol. Cell. Proteomics 8:1751-1764(2009).

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