(Mouse) Nr4a2 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

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AP21233c staining (Mouse) Nr4a2 in mouse brain sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Anti-Nr4a2 Antibody (Center)at 1:2000 dilution + mouse pancreas lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 67 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	Q06219
Reactivity	Human, Rat, Mouse
Host	Rabbit
Clonality	polyclonal
Isotype	Rabbit IgG
Calculated MW	66593 Da

Additional Information
Gene ID	18227
Target/Specificity	This mouse Nr4a2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 164-197 amino acids from the Central region of mouse Nr4a2.
Dilution	WB~~1:2000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	(Mouse) Nr4a2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	Nr4a2
Synonyms	Nurr1
Function	Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development (PubMed:19144721). It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons (PubMed:19144721).
Cellular Location	Cytoplasm {ECO:0000250\|UniProtKB:P43354}. Nucleus {ECO:0000250\|UniProtKB:P43354}. Note=Mostly nuclear; oxidative stress promotes cytoplasmic localization. {ECO:0000250\|UniProtKB:P43354}
Tissue Location	Brain.