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VDAC3 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

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Immunohistochemical analysis of AP17092c on paraffin-embedded Human heart tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded human testis tissue using AP17092c performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Overlay histogram showing Hela cells stained with AP17092c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP17092c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Anti-VDAC3 Antibody (Center) at 1:1000 dilution + NCI-H460 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

产品详情
文献引用 : 1
实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, WB, FC, IHC-P-Leica, E
Primary Accession	Q9Y277
Other Accession	Q9TT13, Q29380, Q60931, Q9MZ13, NP_005653.3, NP_001129166.1
Reactivity	Human, Rat, Mouse
Predicted	Bovine, Mouse, Pig, Rabbit
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	30659 Da
Antigen Region	156-183 aa

Additional Information
Gene ID	7419
Other Names	Voltage-dependent anion-selective channel protein 3, VDAC-3, hVDAC3, Outer mitochondrial membrane protein porin 3, VDAC3
Target/Specificity	This VDAC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-183 amino acids from the Central region of human VDAC3.
Dilution	IHC-P~~1:100 WB~~1:1000 FC~~1:25 IHC-P-Leica~~1:500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	VDAC3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	VDAC3 (HGNC:12674)
Function	Non-selective voltage-gated ion channel that mediates the transport of anions and cations through the mitochondrion outer membrane and plasma membrane (PubMed:31935282). Forms a high-conducting channel with a stable open state and a voltage-induced closure with a mild preference for anions over cations (PubMed:31935282). Involved in male fertility and sperm mitochondrial sheath formation (By similarity).
Cellular Location	Mitochondrion outer membrane {ECO:0000250\|UniProtKB:P21796}. Membrane Note=May localize to non-mitochondrial membranes
Tissue Location	Expressed in erythrocytes (at protein level) (PubMed:27641616). Widely expressed. Highest in testis (PubMed:9781040).

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

VDAC3 belongs to a group of mitochondrial membrane channels involved in translocation of adenine nucleotides through the outer membrane. These channels may also function as a mitochondrial binding site for hexokinase (see HK1; MIM 142600) and glycerol kinase (GK; MIM 300474) (Rahmani et al., 1998).[supplied by OMIM].

REFERENCES

Reina, S., et al. FEBS Lett. 584(13):2837-2844(2010)
Lefievre, L., et al. Proteomics 7(17):3066-3084(2007)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)

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¥ 1,250.00

Cat# AP17092c

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¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

¥ 4,550.00

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VDAC3 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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