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>   首页   >   产品   >   一抗   >   细胞生物学   >   RNF10 Antibody (N-term)   

RNF10 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - RNF10 Antibody (N-term) AP17072a
    RNF10 Antibody (N-term) (Cat. #AP17072a) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the RNF10 antibody detected the RNF10 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q8N5U6
Other Accession NP_055683.3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 89927 Da
Antigen Region 178-206 aa
Additional Information
Gene ID 9921
Other Names RING finger protein 10, RNF10, KIAA0262, RIE2
Target/Specificity This RNF10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-206 amino acids from the N-terminal region of human RNF10.
Dilution WB~~1:1000
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRNF10 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RNF10 {ECO:0000303|PubMed:10697961, ECO:0000312|HGNC:HGNC:10055}
Function E3 ubiquitin-protein ligase that catalyzes monoubiquitination of 40S ribosomal proteins RPS2/us5 and RPS3/us3 in response to ribosome stalling (PubMed:34348161, PubMed:34469731). Part of a ribosome quality control that takes place when ribosomes have stalled during translation initiation (iRQC): RNF10 acts by mediating monoubiquitination of RPS2/us5 and RPS3/us3, promoting their degradation by the proteasome (PubMed:34348161, PubMed:34469731). Also promotes ubiquitination of 40S ribosomal proteins in response to ribosome stalling during translation elongation (PubMed:34348161). The action of RNF10 in iRQC is counteracted by USP10 (PubMed:34469731). May also act as a transcriptional factor involved in the regulation of MAG (Myelin- associated glycoprotein) expression (By similarity). Acts as a regulator of Schwann cell differentiation and myelination (By similarity).
Cellular Location Cytoplasm. Nucleus {ECO:0000250|UniProtKB:Q5XI59}
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

The protein encoded by this gene contains a ring finger motif, which is known to be involved in protein-protein interactions. The specific function of this protein has not yet been determined. EST data suggests the existence of multiple alternatively spliced transcript variants, however, their full length nature is not known.

REFERENCES

Hoshikawa, S., et al. PLoS ONE 3 (10), E3464 (2008) :
Stelzl, U., et al. Cell 122(6):957-968(2005)
Lin, J., et al. Mol. Cell. Biochem. 275 (1-2), 75-84 (2005) :
Seki, N., et al. J. Hum. Genet. 45(1):38-42(2000)

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