DHRS7C Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

DHRS7C Antibody (Center) (Cat. #AP13788c) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the DHRS7C antibody detected the DHRS7C protein (arrow).
DHRS7C Antibody (Center) (AP13788c)immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DHRS7C Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	A6NNS2
Other Accession	Q8CHS7, Q1RMJ5
Reactivity	Human
Predicted	Bovine, Mouse
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	34878 Da
Antigen Region	116-145 aa

Additional Information
Gene ID	201140
Other Names	Dehydrogenase/reductase SDR family member 7C, 11--, DHRS7C
Target/Specificity	This DHRS7C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-145 amino acids from the Central region of human DHRS7C.
Dilution	WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	DHRS7C Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	DHRS7C (HGNC:32423)
Function	NADH-dependent oxidoreductase which catalyzes the oxidation of all-trans-retinol to all-trans-retinal. Plays a role in the regulation of cardiac and skeletal muscle metabolic functions. Maintains Ca(2+) intracellular homeostasis by repressing Ca(2+) release from the sarcoplasmic reticulum (SR) in myotubes, possibly through local alternations in NAD/NADH or retinol/retinal. Also plays a role in Ca(2+) homeostasis by controlling Ca(2+) overload in the cytosol and the SR in myotubes. Involved in glucose uptake into skeletal muscles and muscle performance by activating PI3K and mTORC2-mediated AKT1 phosphorylation signaling pathways, possibly through the action of its downstream catalytic product all-trans-retinoic acid.
Cellular Location	Sarcoplasmic reticulum membrane {ECO:0000250\|UniProtKB:Q8CHS7}. Note=The N-terminus region encompasses a short hydrophobic sequence bound to the sarcoplasmic reticulum membrane, whereas the C-terminus catalytic domain faces the myoplasm In skeletal muscle, enriched in the longitudinal sarcoplasmic reticulum. {ECO:0000250\|UniProtKB:Q8CHS7}

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.