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>   首页   >   产品   >   一抗   >   细胞生物学   >   TRIM54 Antibody (N-term)   

TRIM54 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - TRIM54 Antibody (N-term) AP13292a
    TRIM54 Antibody (N-term) (Cat. #AP13292a) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the TRIM54 antibody detected the TRIM54 protein (arrow).
  • 14 - TRIM54 Antibody (N-term) AP13292a
    TRIM54 Antibody (N-term) (Cat. #AP13292a)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRIM54 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q9BYV2
Other Accession Q58D15, NP_912730.2, NP_115935.3
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 40301 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 57159
Other Names Tripartite motif-containing protein 54, Muscle-specific RING finger protein, MuRF, Muscle-specific RING finger protein 3, MuRF-3, MuRF3, RING finger protein 30, TRIM54, MURF, MURF3, RNF30
Target/Specificity This TRIM54 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TRIM54.
Dilution WB~~1:1000
IHC-P~~1:100~500
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTRIM54 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TRIM54
Synonyms MURF, MURF3, RNF30
Function May bind and stabilize microtubules during myotubes formation.
Cellular Location Cytoplasm, cytoskeleton. Cytoplasm, myofibril, sarcomere, Z line. Note=Associates with microtubules. Localizes to the Z-lines in skeletal muscles (By similarity).
Tissue Location Specifically expressed in heart and skeletal muscle.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

The protein encoded by this gene contains a RING finger motif and is highly similar to the ring finger proteins RNF28/MURF1 and RNF29/MURF2. In vitro studies demonstrated that this protein, RNF28, and RNF29 form heterodimers, which may be important for the regulation of titin kinase and microtubule-dependent signal pathways in striated muscles. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq].

REFERENCES

Centner, T., et al. J. Mol. Biol. 306(4):717-726(2001)
Spencer, J.A., et al. J. Cell Biol. 150(4):771-784(2000)

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