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LGALS4 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - LGALS4 Antibody (N-term) AP12391a
    Anti-LGALS4 Antibody (N-term) at 1:1000 dilution + HT-29 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - LGALS4 Antibody (N-term) AP12391a
    LGALS4 Antibody (N-term) (Cat. #AP12391a) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the LGALS4 antibody detected the LGALS4 protein (arrow).
  • 4 - LGALS4 Antibody (N-term) AP12391a
    LGALS4 Antibody (N-term) (Cat. #AP12391a) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession P56470
Other Accession NP_006140.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 35941 Da
Antigen Region 70-98 aa
Additional Information
Gene ID 3960
Other Names Galectin-4, Gal-4, Antigen NY-CO-27, L-36 lactose-binding protein, L36LBP, Lactose-binding lectin 4, LGALS4
Target/Specificity This LGALS4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-98 amino acids from the N-terminal region of human LGALS4.
Dilution WB~~1:1000
FC~~1:10~50
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLGALS4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LGALS4
Function Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The expression of this gene is restricted to small intestine, colon, and rectum, and it is underexpressed in colorectal cancer.

REFERENCES

Zimbardi, A.L., et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66 (PT 5), 542-545 (2010) :
Stechly, L., et al. Traffic 10(4):438-450(2009)
Tripodi, D., et al. BMC Med Genomics 2, 65 (2009) :
Oberg, C.T., et al. Bioorg. Med. Chem. Lett. 18(13):3691-3694(2008)
Ideo, H., et al. J. Biol. Chem. 282(29):21081-21089(2007)

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