癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
SREBF2 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | Q12772 |
| Other Accession | NP_004590.2 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 123688 Da |
| Antigen Region | 399-427 aa |
| Gene ID | 6721 |
|---|---|
| Other Names | Sterol regulatory element-binding protein 2, SREBP-2, Class D basic helix-loop-helix protein 2, bHLHd2, Sterol regulatory element-binding transcription factor 2, Processed sterol regulatory element-binding protein 2, SREBF2, BHLHD2, SREBP2 |
| Target/Specificity | This SREBF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 399-427 amino acids from the Central region of human SREBF2. |
| Dilution | WB~~1:1000 IHC-P~~1:100~500 IF~~1:10~50 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SREBF2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SREBF2 {ECO:0000303|PubMed:32322062, ECO:0000312|HGNC:HGNC:11290} |
|---|---|
| Function | [Sterol regulatory element-binding protein 2]: Precursor of the transcription factor form (Processed sterol regulatory element- binding protein 2), which is embedded in the endoplasmic reticulum membrane (PubMed:32322062). Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis (PubMed:32322062). |
| Cellular Location | [Sterol regulatory element-binding protein 2]: Endoplasmic reticulum membrane; Multi- pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle, COPII-coated vesicle membrane; Multi-pass membrane protein. Note=At high sterol concentrations, the SCAP- SREBP is retained in the endoplasmic reticulum (PubMed:32322062). Low sterol concentrations promote recruitment into COPII-coated vesicles and transport of the SCAP-SREBP to the Golgi, where it is processed (PubMed:32322062). |
| Tissue Location | Ubiquitously expressed in adult and fetal tissues. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
SREBPs, including SREBP-1a, SREBP-1c, and SREBP-2, constitute a family of basic helix-loop-helix (bHLH) transcription factors that play a critical role in lipid homeostasis by regulating genes involved in cholesterol and fatty acid metabolism. Each SREBP consists of three domains, including an amino-terminal transcription factor domain of ~480 amino acids, a middle hydrophobic region of ~80 amino acids containing two hydrophobic transmembrane segments, and a carboxy-terminal regulatory domain of ~590 amino acids. SREBP-2 regulates cholesterol synthesis by activating the transcription of genes for HMG-CoA reductase and other enzymes of the cholesterol synthetic pathway. SREBP-2 is ubiquitously detected in various tissues. Under basal conditions SREBP is bound to ER membranes as a glycosylated precursor protein. Upon cholesterol depletion, the protein is cleaved to its active forms (about 50-68 kDa) and translocated into the nucleus to stimulate transcription of genes involved in the uptake and synthesis of cholesterol.
REFERENCES
Gerin, I., et al. J. Biol. Chem. 285(44):33652-33661(2010) Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) Marquart, T.J., et al. Proc. Natl. Acad. Sci. U.S.A. 107(27):12228-12232(2010) Najafi-Shoushtari, S.H., et al. Science 328(5985):1566-1569(2010) Pramfalk, C., et al. J. Lipid Res. 51(6):1354-1362(2010)
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