SPATC1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-SPATC1 Antibody (N-term) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: human testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 62kDa Blocking/Dilution buffer: 5% NFDM/TBST.
SPATC1 Antibody (N-term) (Cat. #AP10622a) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the SPATC1 antibody detected the SPATC1 protein (arrow).
SPATC1 antibody (N-term) (Cat. #AP10622a) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPATC1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	Q76KD6
Other Accession	Q148B6, NP_001127846.1, NP_940974.2
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	62399 Da
Antigen Region	1-30 aa

Additional Information
Gene ID	375686
Other Names	Speriolin, Spermatogenesis and centriole-associated protein 1, Spermatogenesis-associated protein 15, Spermatogenic cell-specific Cdc20-binding protein, SPATC1, SPATA15, SPRN
Target/Specificity	This SPATC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SPATC1.
Dilution	WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	SPATC1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	SPATC1
Synonyms	SPATA15, SPRN
Cellular Location	Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Colocalizes with the centrosomal pericentrin protein PCNT1 (By similarity). Located in the connecting piece of sperm. {ECO:0000250, ECO:0000269\|PubMed:20542897}
Tissue Location	Detected only in testis.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.