GATM Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

GATM Antibody (N-term) (Cat. #AP10442a) western blot analysis in HL-60(lane 1),MDA-MB453(lane 2),K562(lane 3) cell line lysates (35ug/lane).This demonstrates the GATM antibody detected the GATM protein (arrow).
GATM antibody (N-term) (Cat. #AP10442a) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GATM antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	P50440
Other Accession	P50442, P50441, Q9D964, Q4R806, Q6PH19, Q9I9K9, Q2HJ74, NP_001473.1
Reactivity	Human
Predicted	Bovine, Chicken, Zebrafish, Monkey, Mouse, Pig, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	48455 Da
Antigen Region	58-84 aa

Additional Information
Gene ID	2628
Other Names	Glycine amidinotransferase, mitochondrial, L-arginine:glycine amidinotransferase, Transamidinase, GATM, AGAT
Target/Specificity	This GATM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-84 amino acids from the N-terminal region of human GATM.
Dilution	WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	GATM Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	GATM
Synonyms	AGAT
Function	Transamidinase that catalyzes the transfer of the amidino group of L-arginine onto the amino moiety of acceptor metabolites such as glycine, beta-alanine, gamma-aminobutyric acid (GABA) and taurine yielding the corresponding guanidine derivatives (PubMed:16820567, PubMed:27233232, PubMed:36543883, PubMed:3800397). Catalyzes the rate- limiting step of creatine biosynthesis, namely the transfer of the amidino group from L-arginine to glycine to generate guanidinoacetate, which is then methylated by GAMT to form creatine. Provides creatine as a source for ATP generation in tissues with high energy demands, in particular skeletal muscle, heart and brain (Probable) (PubMed:27233232, PubMed:36543883, PubMed:3800397, PubMed:9266688).
Cellular Location	[Isoform 1]: Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side. Note=Probably attached to the outer side of the inner membrane
Tissue Location	Expressed in brain, heart, kidney, liver, lung, salivary gland and skeletal muscle tissue, with the highest expression in kidney. Biallelically expressed in placenta and fetal tissues