CHRNA10

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using CHRNA10 mAb against human CHRNA10 (AA: extra 25-237) recombinant protein. (Expected MW is 50 kDa)
Figure 3:Western blot analysis using CHRNA10 mAb against HEK293 (1) and CHRNA10 (AA: extra 25-237)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Flow cytometric analysis of Hela cells using CHRNA10 mouse mAb (green) and negative control (red).

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	Q9GZZ6
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	4E2D1
Isotype	Mouse IgG2a
Calculated MW	49705 Da
Immunogen	Purified recombinant fragment of human CHRNA10 (AA: extra 25-237) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	57053
Other Names	Neuronal acetylcholine receptor subunit alpha-10, Nicotinic acetylcholine receptor subunit alpha-10, NACHR alpha-10, CHRNA10, NACHRA10
Dilution	WB~~ 1/500 - 1/2000 IHC~~1:100~500 ICC~~N/A E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CHRNA10 is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	CHRNA10 (HGNC:13800)
Synonyms	NACHRA10
Function	Component of neuronal acetylcholine receptors (nAChRs) that function as pentameric, ligand-gated cation channels with high calcium permeability. nAChRs are excitatory neurotrasnmitter receptors formed by a collection of nAChR subunits. Each nAchR subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, cation permeability, and binding to allosteric modulators (Probable). Forms heteropentamers with CHRNA9. Expressed in the inner ear, in sympathetic neurons and in other non-neuronal cells, such as skin keratinocytes and lymphocytes (PubMed:11752216, PubMed:15531379). nAChR formed by CHRNA9:CHRNA10 is involved in modulation of auditory stimuli. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane. In the ear, mediates synaptic transmission between efferent olivocochlear fibers and hair cells of the cochlea, this may lead to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing (PubMed:11752216). This may protect against acoustic trauma. May also regulate keratinocyte adhesion (By similarity).
Cellular Location	Synaptic cell membrane {ECO:0000250\|UniProtKB:Q9JLB5}; Multi-pass membrane protein. Cell membrane {ECO:0000250\|UniProtKB:Q9JLB5}; Multi-pass membrane protein
Tissue Location	Expressed in inner-ear tissue, tonsil, immortalized B-cells, cultured T-cells and peripheral blood lymphocytes