GRIN3B

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using GRIN3B mAb against human GRIN3B (AA: 135-276) recombinant protein. (Expected MW is 40.8 kDa)
Figure 3:Western blot analysis using GRIN3B mAb against HEK293 (1) and GRIN3B (AA: 135-276)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Flow cytometric analysis of SH-SY5Y cells using GRIN3B mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of SK-N-SH cells using GRIN3B mouse mAb (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	O60391
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	2A8E11
Isotype	Mouse IgG2a
Calculated MW	112992 Da
Immunogen	Purified recombinant fragment of human GRIN3B (AA: 135-276) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	116444
Other Names	NR3B; GluN3B
Dilution	WB~~ 1/500 - 1/2000 IHC~~1:100~500 ICC~~N/A E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	GRIN3B is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	GRIN3B (HGNC:16768)
Function	Component of a non-conventional N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with low calcium permeability and low voltage-dependent block by Mg(2+) (By similarity). Forms glutamatergic receptor complexes with GluN1 and GluN2 subunits which are activated by glycine binding to the GluN1 and GluN3 subunits and L-glutamate binding to GluN2 subunits (By similarity). Forms excitatory glycinergic receptor complexes with GluN1 alone which are activated by glycine binding to the GluN1 and GluN3 subunits. GluN3B subunit also binds D-serine and, in the absence of glycine, activates glycinergic receptor complexes, but with lower efficacy than glycine (By similarity). Each GluN3 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (By similarity).
Cellular Location	Cell membrane {ECO:0000250\|UniProtKB:Q91ZU9}; Multi-pass membrane protein {ECO:0000250\|UniProtKB:Q13224} Postsynaptic cell membrane {ECO:0000250\|UniProtKB:Q91ZU9} Note=Requires the presence of GRIN1 to be targeted at the plasma membrane. {ECO:0000250\|UniProtKB:Q91ZU9}

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.