注册 | 登录
点击这里给我发消息
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   其他   >   Mouse Monoclonal Antibody to C1QA   

Mouse Monoclonal Antibody to C1QA

Purified Mouse Monoclonal Antibody

     
  • 10 - Mouse Monoclonal Antibody to C1QA AO2438a
    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • 1 - Mouse Monoclonal Antibody to C1QA AO2438a
    Western blot analysis using C1QA mAb against human C1QA (AA: 23-167) recombinant protein. (Expected MW is 40.6 kDa)
  • 1 - Mouse Monoclonal Antibody to C1QA AO2438a
    Western blot analysis using C1QA mAb against HEK293 (1) and C1QA (AA: 23-167)-hIgGFc transfected HEK293 (2) cell lysate.
  • 4 - Mouse Monoclonal Antibody to C1QA AO2438a
    Flow cytometric analysis of Hela cells using C1QA mouse mAb (green) and negative control (red).
  • 2 - Mouse Monoclonal Antibody to C1QA AO2438a
    Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using C1QA mouse mAb with DAB staining.
  • 产品详情
  • 实验流程
Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC, FC, E
Primary Accession P02745
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1C7G3
Isotype Mouse IgG2b
Calculated MW 26017 Da
Description This gene encodes a major constituent of the human complement subcomponent C1q. C1q associates with C1r and C1s in order to yield the first component of the serum complement system. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. The A-, B-, and C-chains are arranged in the order A-C-B on chromosome 1. This gene encodes the A-chain polypeptide of human complement subcomponent C1q.;
Immunogen Purified recombinant fragment of human C1QA (AA: 23-167) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application Note ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; FCM: 1/200 - 1/400
Additional Information
Gene ID 712
Other Names Complement C1q subcomponent subunit A, C1QA
Dilution WB~~1:1000
IHC~~1:100~500
FC~~1:10~50
E~~N/A
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Monoclonal Antibody to C1QA is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name C1QA {ECO:0000303|PubMed:1706597, ECO:0000312|HGNC:HGNC:1241}
Function Core component of the complement C1 complex, a multiprotein complex that initiates the classical pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed:12847249, PubMed:19006321, PubMed:24626930, PubMed:29449492, PubMed:3258649, PubMed:34155115, PubMed:6249812, PubMed:6776418). The classical complement pathway is initiated by the C1Q subcomplex of the C1 complex, which specifically binds IgG or IgM immunoglobulins complexed with antigens, forming antigen-antibody complexes on the surface of pathogens: C1QA, together with C1QB and C1QC, specifically recognizes and binds the Fc regions of IgG or IgM via its C1q domain (PubMed:12847249, PubMed:19006321, PubMed:24626930, PubMed:29449492, PubMed:3258649, PubMed:6776418). Immunoglobulin-binding activates the proenzyme C1R, which cleaves C1S, initiating the proteolytic cascade of the complement system (PubMed:29449492). The C1Q subcomplex is activated by a hexamer of IgG complexed with antigens, while it is activated by a pentameric IgM (PubMed:19706439, PubMed:24626930, PubMed:29449492). The C1Q subcomplex also recognizes and binds phosphatidylserine exposed on the surface of cells undergoing programmed cell death, possibly promoting activation of the complement system (PubMed:18250442).
Cellular Location Secreted. Cell surface. Note=Specifically binds IgG or IgM immunoglobulins complexed with antigens, forming antigen-antibody complexes on the surface of pathogens.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

REFERENCES

1.Immunology. 2012 May;136(1):78-85. ; 2.Br J Cancer. 2010 Apr 13;102(8):1294-9.;

FeedBack

终于等到您。ABCEPTA(百远生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-88856768 tech-china@abcepta.com.


我要评价