CBX8 Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 1: Western blot analysis using CBX8 mAb against human CBX8 recombinant protein. (Expected MW is 49.5 kDa)
Figure 2: Western blot analysis using CBX8 mAb against HEK293 (1) and CBX8 (AA: 17-222)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3: Flow cytometric analysis of HEK293 cells using CBX8 mouse mAb (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, E
Primary Accession	Q9HC52
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	9C3D4
Isotype	IgG2b
Calculated MW	43396 Da
Description	Chromobox homolog 8 (CBX8), a Polycomb Group protein that interacts with MLL-AF9 and TIP60, plays an essential role in MLL-AF9 transcriptional regulation and leukemogenesis. CBX8, which is part of one of the PRC1 complexes, regulates proliferation of diploid human and mouse fibroblasts through direct binding to the INK4A-ARF locus.
Immunogen	Purified recombinant fragment of human CBX8 (AA: 17-222 ) expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Additional Information
Gene ID	57332
Other Names	Chromobox protein homolog 8, Polycomb 3 homolog, Pc3, hPc3, Rectachrome 1, CBX8, PC3, RC1
Dilution	WB~~1/500 - 1/2000 FC~~1/200 - 1/400 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CBX8 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	CBX8
Synonyms	PC3, RC1
Function	Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
Cellular Location	Nucleus.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.