TBCC Antibody

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using TBCC mAb against human TBCC (AA: 1-196) recombinant protein. (Expected MW is 47.9 kDa)
Figure 3:Western blot analysis using TBCC mAb against HEK293 (1) and TBCC (AA: 1-196)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Immunofluorescence analysis of Hela cells using TBCC mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using TBCC mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using TBCC mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	Q15814
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	7G6H1
Isotype	IgG1
Calculated MW	39248 Da
Description	Cofactor C is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state.
Immunogen	Purified recombinant fragment of human *** (AA: 1-196) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	6903
Other Names	Tubulin-specific chaperone C, Tubulin-folding cofactor C, CFC, TBCC
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 ICC~~N/A E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	TBCC Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	TBCC
Function	Tubulin-folding protein; involved in the final step of the tubulin folding pathway.
Cellular Location	Cytoplasm. Note=Detected predominantly in the photoreceptor connecting cilium
Tissue Location	Expressed in the retina. Expressed in the rod and cone photoreceptors, extending from the inner segments (IS), through the outer nuclear layer (ONL) and into the synapses in the outer plexiform layer (OPL). Strongly expressed to the photoreceptor connecting cilium at the tips of the IS (at protein level)

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.