注册 | 登录
点击这里给我发消息
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   其他   >   PRDM1 Antibody   

PRDM1 Antibody

Purified Mouse Monoclonal Antibody

     
  • 10 - PRDM1 Antibody AO2132a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • 1 - PRDM1 Antibody AO2132a

    Figure 2:Western blot analysis using PRDM1 mAb against human PRDM1 (AA: 690-825) recombinant protein. (Expected MW is 40.3 kDa)

  • 1 - PRDM1 Antibody AO2132a

    Figure 3:Western blot analysis using PRDM1 mAb against HEK293 (1) and PRDM1 (AA: 690-825)-hIgGFc transfected HEK293 (2) cell lysate.

  • 3 - PRDM1 Antibody AO2132a

    Figure 4:Immunofluorescence analysis of HeLa cells using PRDM1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • 4 - PRDM1 Antibody AO2132a

    Figure 5:Flow cytometric analysis of Raji cells using PRDM1 mouse mAb (green) and negative control (red).

  • 产品详情
  • 实验流程
Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, ICC, E
Primary Accession O75626
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2F1B6
Isotype IgG2b
Calculated MW 91771 Da
Description This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported.
Immunogen Purified recombinant fragment of human PRDM1 (AA: 690-825) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 639
Other Names PR domain zinc finger protein 1, 2.1.1.-, BLIMP-1, Beta-interferon gene positive regulatory domain I-binding factor, PR domain-containing protein 1, Positive regulatory domain I-binding factor 1, PRDI-BF1, PRDI-binding factor 1, PRDM1, BLIMP1
Dilution WB~~1/500 - 1/2000
FC~~1/200 - 1/400
ICC~~N/A
E~~1/10000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPRDM1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PRDM1
Synonyms BLIMP1
Function Transcription factor that mediates a transcriptional program in various innate and adaptive immune tissue-resident lymphocyte T cell types such as tissue-resident memory T (Trm), natural killer (trNK) and natural killer T (NKT) cells and negatively regulates gene expression of proteins that promote the egress of tissue-resident T-cell populations from non-lymphoid organs. Plays a role in the development, retention and long-term establishment of adaptive and innate tissue- resident lymphocyte T cell types in non-lymphoid organs, such as the skin and gut, but also in other nonbarrier tissues like liver and kidney, and therefore may provide immediate immunological protection against reactivating infections or viral reinfection (By similarity). Binds specifically to the PRDI element in the promoter of the beta- interferon gene (PubMed:1851123). Drives the maturation of B- lymphocytes into Ig secreting cells (PubMed:12626569). Associates with the transcriptional repressor ZNF683 to chromatin at gene promoter regions (By similarity). Binds to the promoter and acts as a transcriptional repressor of IRF8, thereby promotes transcription of osteoclast differentiation factors such as NFATC1 and EEIG1 (By similarity).
Cellular Location Nucleus. Cytoplasm
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

REFERENCES

1.Proc Natl Acad Sci U S A. 2011 Dec 13;108(50):20119-24.2.Mol Cancer Res. 2010 Jun;8(6):907-18.

FeedBack

终于等到您。ABCEPTA(百远生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-88856768 tech-china@abcepta.com.


我要评价