THAP1 Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 2:Western blot analysis using THAP1 mAb against human THAP1 (AA: 1-213) recombinant protein. (Expected MW is 50.3 kDa)
Figure 3:Western blot analysis using THAP1 mAb against HEK293 (1) and THAP1 (AA: 1-213)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Flow cytometric analysis of A549 cells using THAP1 mouse mAb (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, E
Primary Accession	Q9NVV9
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	2F1B4
Isotype	IgG1
Calculated MW	24944 Da
Description	The protein encoded by this gene contains a THAP domain, a conserved DNA-binding domain. This protein colocalizes with the apoptosis response protein PAWR/PAR-4 in promyelocytic leukemia (PML) nuclear bodies, and functions as a proapoptotic factor that links PAWR to PML nuclear bodies. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Immunogen	Purified recombinant fragment of human THAP1 (AA: 1-213) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	55145
Other Names	THAP domain-containing protein 1, THAP1
Dilution	WB~~1/500 - 1/2000 FC~~1/200 - 1/400 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	THAP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	THAP1
Function	DNA-binding transcription regulator that regulates endothelial cell proliferation and G1/S cell-cycle progression. Specifically binds the 5'-[AT]NTNN[GT]GGCA[AGT]-3' core DNA sequence and acts by modulating expression of pRB-E2F cell-cycle target genes, including RRM1. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. May also have pro-apoptotic activity by potentiating both serum- withdrawal and TNF-induced apoptosis.
Cellular Location	Nucleus, nucleoplasm. Nucleus, PML body
Tissue Location	Highly expressed in heart, skeletal muscle, kidney and liver. Weaker expression in brain and placenta

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.