TIE1 Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 1: Western blot analysis using TIE1 mAb against human TIE1 (AA: 385-607) recombinant protein. (Expected MW is 50.6 kDa)
Figure 2: Western blot analysis using TIE1 mAb against HEK293 (1) and TIE1 (AA: 385-607)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3: Western blot analysis using TIE1 mouse mAb against HepG2 cell lysate.
Figure 4: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using TIE1 mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded kidney tissues using TIE1 mouse mAb with DAB staining.

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实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, E
Primary Accession	P35590
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	8D12D2
Isotype	IgG1
Calculated MW	125090 Da
Description	This gene encodes a member of the tyrosine protein kinase family. The encoded protein plays a critical role in angiogenesis and blood vessel stability by inhibiting angiopoietin 1 signaling through the endothelial receptor tyrosine kinase Tie2. Ectodomain cleavage of the encoded protein relieves inhibition of Tie2 and is mediated by multiple factors including vascular endothelial growth factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Immunogen	Purified recombinant fragment of human TIE1 (AA: 385-607) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide.

Additional Information
Gene ID	7075
Other Names	Tyrosine-protein kinase receptor Tie-1, 2.7.10.1, TIE1, TIE
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	TIE1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	TIE1
Synonyms	TIE
Function	Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.
Cellular Location	Cell membrane; Single-pass type I membrane protein
Tissue Location	Specifically expressed in developing vascular endothelial cells.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

This locus encodes the transforming growth factor (TGF)-beta type III receptor. The encoded receptor is a membrane proteoglycan that often functions as a co-receptor with other TGF-beta receptor superfamily members. Ectodomain shedding produces soluble TGFBR3, which may inhibit TGFB signaling. Decreased expression of this receptor has been observed in various cancers. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. ; ; ;

REFERENCES

1. Int J Oncol. 2007 Oct;31(4):893-7. 2. Cancer. 2002 Mar 1;94(5):1517-21.

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¥ 2,600.00

Cat# AO1918a

Size:

100 µl

Price:

¥ 2,600.00

Quantity:

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Availability: 3-5 days

询价

TIE1 Antibody

Purified Mouse Monoclonal Antibody

BACKGROUND

REFERENCES

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