THY1 Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 1: Western blot analysis using THY1 mAb against human THY1 recombinant protein. (Expected MW is 38.5 kDa)
Figure 2: Western blot analysis using THY1 mAb against HEK293 (1) and THY1 (AA: 17-132)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3: Western blot analysis using THY1 mouse mAb against T47D (1), HepG2 (2) and PC-12 (3) cell lysate.
Figure 4: Immunofluorescence analysis of Hela cells using THY1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 5: Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using THY1 mouse mAb with DAB staining.
Figure 6: Immunohistochemical analysis of paraffin-embedded cerebellum tissues using THY1 mouse mAb with DAB staining.

产品详情
实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	P04216
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	7E1B11
Isotype	IgG1
Calculated MW	17935 Da
Description	THY1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain
Immunogen	Purified recombinant fragment of human THY1 (AA: 17-132) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Other Names	Thy-1 membrane glycoprotein, CDw90, Thy-1 antigen, CD90, THY1
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 ICC~~N/A E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	THY1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described. ; ;

REFERENCES

1. Acta Histochem. 2011 Dec;113(8):833-8. 2. J Immunol. 2012 Feb 1;188(3):981-91.

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¥ 1,500.00

Cat# AO1848a

Size:

50 µl

100 µl

Price:

¥ 1,500.00

¥ 2,600.00

Quantity:

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Availability: 3-5 days

询价

THY1 Antibody

Purified Mouse Monoclonal Antibody

BACKGROUND

REFERENCES

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