PRKACG Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 1: Western blot analysis using PRKACG mAb against human PRKACG (AA: 164-351) recombinant protein. (Expected MW is 47.1 kDa)
Figure 2: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using PRKACG mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PRKACG mouse mAb with DAB staining.
Figure 4: Flow cytometric analysis of MCF-7 cells using PRKACG mouse mAb (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, E
Primary Accession	P22612
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	2E4
Isotype	IgG1
Calculated MW	40434 Da
Description	Cyclic AMP-dependent protein kinase (PKA) consists of two catalytic subunits and a regulatory subunit dimer. This gene encodes the gamma form of its catalytic subunit. The gene is intronless and is thought to be a retrotransposon derived from the gene for the alpha form of the PKA catalytic subunit.
Immunogen	Purified recombinant fragment of human PRKACG expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Additional Information
Gene ID	5568
Other Names	cAMP-dependent protein kinase catalytic subunit gamma, PKA C-gamma, 2.7.11.11, PRKACG
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 FC~~1/200 - 1/400 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	PRKACG Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	PRKACG
Function	Phosphorylates a large number of substrates in the cytoplasm and the nucleus.
Tissue Location	Testis specific. But important tissues such as brain and ovary have not been analyzed for the content of transcript

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.