ETS1 Antibody

Purified Mouse Monoclonal Antibody

Figure 1: Western blot analysis using ETS1 mouse mAb against Jurkat (1), HepG2 (2) and ETS1-hIgGFc transfected HEK293 (3) cell lysate.
Figure 2: Immunohistochemical analysis of paraffin-embedded human Colon, Adipocytes tissues using anti-ETS1 mouse mAb
Figure 3: Immunofluorescence analysis of NIH/3T3 cells using anti-ETS1 mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Figure 4: Flow cytometric analysis of Jurkat cells using anti-ETS1 mAb (green) and negative control (purple).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, ICC, E
Primary Accession	P14921
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	8A8
Isotype	IgG1
Calculated MW	50408 Da
Description	The ETS1 is a transcription factor known to regulate expression of a number of genes involved in extracellular matrix remodeling. ETS1 is absent from normal gastric epithelium but is expressed in approximately 60% of gastric carcinomas and oral squamous cell carcinomas with a significant correlation to the tumor stage.
Immunogen	Purified recombinant fragment of human ETS1 expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Additional Information
Gene ID	2113
Other Names	Protein C-ets-1, p54, ETS1, EWSR2
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 FC~~1/200 - 1/400 ICC~~N/A E~~N/A
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	ETS1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	ETS1
Synonyms	EWSR2
Function	Transcription factor (PubMed:10698492, PubMed:11909962). Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts (PubMed:20378371). May control the differentiation, survival and proliferation of lymphoid cells (PubMed:20378371). May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion (PubMed:15247905, PubMed:15592518).
Cellular Location	Nucleus. Cytoplasm Note=Delocalizes from nucleus to cytoplasm when coexpressed with isoform Ets-1 p27.
Tissue Location	Highly expressed within lymphoid cells. Isoforms c- ETS-1A and Ets-1 p27 are both detected in all fetal tissues tested, but vary with tissue type in adult tissues. None is detected in brain or kidney.