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ALDOC Antibody (C-term)

Mouse Monoclonal Antibody (Mab)

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Overlay histogram showing HL-60 cells stained with AM2215B(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM2215B, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG3 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
All lanes : Anti-ALDOC Antibody (C-term) at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: 293T/17 whole cell lysate Lane 3: mouse brain lysate Lane 4: rat brain lysate Lane 5: mouse spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, E
Primary Accession	P09972
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Isotype	IgG3
Clone Names	859CT9.5.3
Calculated MW	39456 Da

Additional Information
Gene ID	230
Other Names	Fructose-bisphosphate aldolase C, Brain-type aldolase, ALDOC, ALDC
Target/Specificity	Purified His-tagged ALDOC protein was used to produced this monoclonal antibody.
Dilution	WB~~1:2000 FC~~1:25 E~~Use at an assay dependent concentration.
Format	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	ALDOC Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	ALDOC
Synonyms	ALDC

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Rottmann W.H., et al. Biochimie 69:137-145(1987).
Buono P., et al. Nucleic Acids Res. 16:4733-4733(1988).
Buono P., et al. Eur. J. Biochem. 192:805-811(1990).
Yu W., et al. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
Kalnine N., et al. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.

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¥ 1,250.00

Cat# AM2215b

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¥ 2,050.00

¥ 2,960.00

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ALDOC Antibody (C-term)

Mouse Monoclonal Antibody (Mab)

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