Goat anti-CMG1 / CCDC2 / IFT74, Biotinylated Antibody

Peptide-affinity purified goat antibody

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, Pep-ELISA
Primary Accession	Q96LB3
Other Accession	NP_001092692.1, NP_001092693.1, NP_079379.2, AAK77221.1
Reactivity	Human, Rat
Host	Goat
Clonality	Polyclonal
Clone Names	IFT74
Calculated MW	69239 Da

Additional Information
Gene ID	80173
Other Names	IFT74; CCDC2; CMG-1; FLJ22621; MGC111562; intraflagellar transport 74 homolog (Chlamydomonas); capillary morphogenesis protein 1; coiled-coil domain containing 2; CMG1
Dilution	WB~~1:1000 Pep-ELISA~~N/A
Format	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	Goat anti-CMG1 / CCDC2 / IFT74, Biotinylated Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	IFT74
Synonyms	CCDC2, CMG1
Function	Component of the intraflagellar transport (IFT) complex B: together with IFT81, forms a tubulin-binding module that specifically mediates transport of tubulin within the cilium (PubMed:23990561). Binds beta-tubulin via its basic region (PubMed:23990561). Required for ciliogenesis (PubMed:23990561). Essential for flagellogenesis during spermatogenesis (PubMed:33689014).
Cellular Location	Cell projection, cilium. Cytoplasmic vesicle. Cell projection, cilium, flagellum. Cytoplasmic vesicle, secretory vesicle, acrosome {ECO:0000250\|UniProtKB:Q8BKE9}. Note=Localizes along primary cilia at interphase and around the basal body/centriole at interphase and mitosis (PubMed:15024030). In male germ cells, strongly expressed in the vesicles of spermatocytes and round spermatids and also in the acrosome and centrosome regions of elongating spermatids and in developing sperm tails (By similarity). {ECO:0000250\|UniProtKB:Q8BKE9, ECO:0000269\|PubMed:15024030}
Tissue Location	Highly expressed in adult and fetal kidney and expressed at lower level in adult heart, placenta, lung, liver and pancreas, and in fetal heart, lung and liver. Little to no expression was detected in adult brain and skeletal muscle or in fetal brain, thymus and spleen (PubMed:11683410). Detected in sperm (at protein level) (PubMed:33689014).