5-color Multiplex IHC Kit（520 570 620 690 DAPI）

Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

Tyramide Signal Amplification (TSA) is an advanced technique employed in immunofluorescence and other enzyme-mediated applications to dramatically enhance signal detection. The principle of TSA revolves around the catalytic activity of horseradish peroxidase (HRP), which, in the presence of hydrogen peroxide, facilitates the deposition of tyramide molecules that are conjugated to fluorescent dyes. This localized amplification allows for the visualization of low-abundance targets with improved sensitivity and resolution. Multiplex immunofluorescence using TSA enables the simultaneous detection of multiple antigens within the same sample. This approach is particularly advantageous when working with limited biological samples or when seeking comprehensive profiling of cellular phenotypes. The TSA-based multiplex immunofluorescence kits are designed to streamline the workflow of multi-target detection. These kits typically include a set of optimized reagents: antibodies labeled with HRP, tyramide-fluorophore conjugates, blocking solutions, and buffers. By combining the specificity of antibodies with the potent signal amplification of TSA, researchers can achieve distinct and vibrant labeling of several antigens in situ. This methodology has broad applications across various fields of biomedical research, including oncology, neuroscience, and developmental biology. Specifically, it facilitates the elucidation of complex cellular pathways, characterization of heterogeneous cell populations, and advancement of diagnostic assays. The ability to co-localize different proteins within subcellular compartments enhances our understanding of cellular function and disease pathology. Overall, TSA-based multiplex immunofluorescence kits represent a powerful toolset for precise and sensitive biomarker analysis, contributing significantly to the expanding capabilities in tissue imaging and molecular diagnostics.