癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
TRIM25 Antibody (monoclonal) (M03)
Mouse monoclonal antibody raised against a partial recombinant TRIM25.
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IHC, IF |
|---|---|
| Primary Accession | Q14258 |
| Other Accession | NM_005082 |
| Reactivity | Human |
| Host | mouse |
| Clonality | monoclonal |
| Isotype | IgG1 Kappa |
| Clone Names | 5C3 |
| Calculated MW | 70973 Da |
| Gene ID | 7706 |
|---|---|
| Other Names | E3 ubiquitin/ISG15 ligase TRIM25, 632n3, Estrogen-responsive finger protein, RING finger protein 147, Tripartite motif-containing protein 25, Ubiquitin/ISG15-conjugating enzyme TRIM25, Zinc finger protein 147, TRIM25, EFP, RNF147, ZNF147 |
| Target/Specificity | TRIM25 (NP_005073, 211 a.a. ~ 310 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 IHC~~1:100~500 IF~~1:50~200 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | TRIM25 Antibody (monoclonal) (M03) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.
REFERENCES
The role of height-associated loci identified in genome wide association studies in the determination of pediatric stature. Zhao J, et al. BMC Med Genet, 2010 Jun 14. PMID 20546612.Expression of Efp, VEGF and bFGF in normal, hyperplastic and malignant endometrial tissue. Dai H, et al. Oncol Rep, 2010 Mar. PMID 20127022.Influenza A virus NS1 targets the ubiquitin ligase TRIM25 to evade recognition by the host viral RNA sensor RIG-I. Gack MU, et al. Cell Host Microbe, 2009 May 8. PMID 19454348.Influenza A virus TRIMs the type I interferon response. Ludwig S, et al. Cell Host Microbe, 2009 May 8. PMID 19454344.Regulation of the vascular endothelial growth factor and growth by estrogen and antiestrogens through Efp in Ishikawa endometrial carcinoma cells. Dai H, et al. Oncol Rep, 2009 Feb. PMID 19148513.
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