Anti-Mouse IgG (H&L) (Peroxidase Conjugated) Secondary Antibody
Donkey Polyclonal, Peroxidase (Horseradish)
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| Description | Anti-MOUSE IgG (H&L) (DONKEY) Antibody Peroxidase Conjugated |
|---|---|
| Host | Donkey |
| Conjugate | Peroxidase (Horseradish) |
| Target Species | Mouse |
| Reactivity | Mouse |
| Clonality | Polyclonal |
Application
| WB |
| Physical State | Lyophilized |
| Host Isotype | IgG |
| Target Isotype | IgG (H&L) |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in donkey |
| Reconstitution Volume | 1.0 mL |
| Reconstitution Buffer | Restore with deionized water (or equivalent) |
| Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Shipping Condition | Ambient |
|---|---|
| Application Note | Antibody anti mouse peroxidase conjugated has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product. Antibody anti mouse peroxidase conjugated has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product. |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Mouse IgG and Mouse Serum. |
| Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Anti-Mouse IgG (H&L) peroxidase conjugated antibody generated in donkey detects specifically mouse IgG (H&L). This secondary peroxidase conjugated antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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