RFK Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of RFK (arrow) using rabbit polyclonal RFK Antibody (C15) (Cat. #AP7183a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RFK gene.
RFK Antibody (N-term) (Cat. #AP7183a) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, WB, FC, E
Primary Accession	Q969G6
Other Accession	Q8CFV9
Reactivity	Human
Predicted	Mouse
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	17623 Da
Antigen Region	1-30 aa

Additional Information
Gene ID	55312
Other Names	Riboflavin kinase, ATP:riboflavin 5'-phosphotransferase, Flavokinase, RFK
Target/Specificity	This RFK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human RFK.
Dilution	IHC-P~~1:100~500 WB~~1:1000 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	RFK Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	RFK
Function	Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF- activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase.
Cellular Location	Cytoplasm.
Tissue Location	Detected in brain, placenta and urinary bladder.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.