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Phospho-TNIK(S764) Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Dot blot analysis of anti-TNIK(S764) Antibody Phospho-specific Pab (Cat. #AP3276a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0. 5ug per ml.
Dot blot analysis of Phospho-TNIK-pS764 Pab (Cat. #AP3276a) and TNIK-pS764 Pab (AP7970d)on nitrocellulose membrane. 50ng of Phospho-peptide (BP3276a) or Non Phospho-peptide (BP7970d) per dot were adsorbed. Antobodies working concentration was 0.5ug per ml. P-Pab: phosphorylated antibody; NP-Pab: non-phophorylated antibody; P-Pep: phospho-peptide; NP-Pep: non-phospho-peptide.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

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实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, DB, IHC-P, E
Primary Accession	Q9UKE5
Reactivity	Human, Rat, Mouse
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG

Additional Information
Other Names	TRAF2 and NCK-interacting protein kinase, TNIK, KIAA0551
Target/Specificity	This TNIK Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S764 of human TNIK.
Dilution	WB~~1:1000 DB~~1:500 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	Phospho-TNIK(S764) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

TNIK is a stress-activated serine/threonine kinase that may play a role in the response to environmental stress. This protein appears to act upstream of the JUN N-terminal pathway, and may play a role in cytoskeletal regulation.

REFERENCES

Taira, K., et al., J. Biol. Chem. 279(47):49488-49496 (2004).
Fu, C.A., et al., J. Biol. Chem. 274(43):30729-30737 (1999).
Yonekura, H., et al., Nucleic Acids Res. 27(13):2591-2600 (1999).

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¥ 1,250.00

Cat# AP3276A

Size:

50 µl

100 µl

200 µl

400 µl

Price:

¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

¥ 4,550.00

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Phospho-TNIK(S764) Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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