RN185 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

RN185 Antibody (Center) (Cat. #AP10818c) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the RN185 antibody detected the RN185 protein (arrow).
RN185 Antibody (Center) (Cat. #AP10818c) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
RN185 Antibody (Center) (Cat. #AP10818c) western blot analysis in mouse kidney tissue and K562 cell line lysates (35ug/lane).This demonstrates the RN185 antibody detected the RN185 protein (arrow).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, E
Primary Accession	Q96GF1
Other Accession	Q568Y3, Q91YT2, Q6PC78, Q5ZIR9, NP_689480.2
Reactivity	Human, Hamster, Mouse
Predicted	Chicken, Zebrafish, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	20459 Da
Antigen Region	88-117 aa

Additional Information
Gene ID	91445
Other Names	E3 ubiquitin-protein ligase RNF185, 632-, RING finger protein 185, RNF185
Target/Specificity	This RN185 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-117 amino acids from the Central region of human RN185.
Dilution	WB~~1:1000 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	RN185 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	RNF185 {ECO:0000303\|Ref.1, ECO:0000312\|HGNC:HGNC:26783}
Function	E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating 'Lys-63'-linked polyubiquitination of BNIP1 (PubMed:21931693). Acts in the endoplasmic reticulum (ER)- associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for ubiquitination and subsequent proteasome-mediated degradation (PubMed:27485036). Protects cells from ER stress-induced apoptosis (PubMed:27485036). Responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway (PubMed:24019521). Also acts as a regulator of the innate antiviral response by catalyzing 'Lys-27'-linked polyubiquitination of CGAS at 'Lys-173' and 'Lys-384', thereby promoting CGAS cyclic GMP-AMP synthase activity (PubMed:28273161). Preferentially associates with the E2 enzymes UBE2J1 and UBE2J2 (PubMed:24019521).
Cellular Location	Mitochondrion outer membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein
Tissue Location	Ubiquitously expressed.