癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
HACE1 Antibody [Knockdown Validated]
Purified Mouse Monoclonal Antibody (Mab)
![1 - HACE1 Antibody [Knockdown Validated] AM8644b](/assets/uploads/products/201612/AM8644b_WB_1.jpg)
![1 - HACE1 Antibody [Knockdown Validated] AM8644b](/assets/uploads/products/202511/B8644b101.jpg)
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | Q8IYU2 |
| Reactivity | Human, Mouse, Rat |
| Host | Mouse |
| Clonality | monoclonal |
| Isotype | IgG1,k |
| Clone Names | 1854CT757.66.47 |
| Calculated MW | 102342 Da |
| Gene ID | 57531 |
|---|---|
| Other Names | E3 ubiquitin-protein ligase HACE1, 6.3.2.-, HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1, HACE1, KIAA1320 |
| Target/Specificity | This HACE1 antibody is generated from a mouse immunized with a recombinant protein from the human region of human HACE1. |
| Dilution | WB~~1:2000 E~~Use at an assay dependent concentration. |
| Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | HACE1 Antibody [Knockdown Validated] is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | HACE1 |
|---|---|
| Synonyms | KIAA1320 |
| Function | E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases (PubMed:15254018, PubMed:21988917, PubMed:22036506, PubMed:37537642, PubMed:38332367). Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion (PubMed:21988917). Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division (PubMed:21988917). Specifically binds GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens (PubMed:22036506, PubMed:37537642, PubMed:38332367). May also act as a transcription regulator via its interaction with RARB (By similarity). |
| Cellular Location | Golgi apparatus, Golgi stack membrane. Cytoplasm Endoplasmic reticulum. Note=A significant portion localizes to the endoplasmic reticulum. Targeted to Golgi membrane via its interaction with Rab proteins |
| Tissue Location | Expressed in multiple tissues including heart, brain and kidney. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB.
REFERENCES
Nagase T.,et al.DNA Res. 7:65-73(2000).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mungall A.J.,et al.Nature 425:805-811(2003).
Anglesio M.S.,et al.Hum. Mol. Genet. 13:2061-2074(2004).
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